We've been planning to switch to a bioreactor to increase our protein yields. However virtually every single protocol I find on them uses Pichia not Saccharomyces. Does anyone have suggestions on how to go about adapting protocols? Obviously for adapting I'm going to start with our original protocol that used an orbital shaker, so I'm not completely starting from scratch, but still there seems to be a dearth of information on this sort of expression.
Run some trial after obtaining and satisfying the results you can then scale it up.
I do not understand the difficulty with finding papers on Pichia fermentation. For example, Eppendorf Application Note 253.
The manufacturer of your bioreactor probably has something similar if it is not Eppendorf.
Anyway, do not start with a shake flask protocol, the growth conditions are completely different in a bioreactor.
Once you can grow a decent amount of cells in your bioreactor system then you can adjust some basic parameters to see if your yield increases.
Start with simple things like pH, temperature, dissolved oxygen (DO), cell density at induction (measured by optical density or wet cell weight typically), carbon source type. Then you can adjust individual media components. Remember that you can have completely different conditions before and after induction.
Using 3-5 factors in a standard Design of Experiment can be useful once you have established the range of each parameter.
Also keep in mind that if you are using yeast extract, the type of yeast extract (brand and part number within the same brand) can be very important. The amount of hydrolysis and the source of the yeast extract can impact the yield significantly.
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