I want to measure stomatal size (length) in order to correlate it to ploidy. Now I remember from plant physiology classes long ago, that stomata become shorter and wider when they open. It seems therefore, that the momentanous condition of the plant matters when the imprint is made.
In the methods section of taxonomic literature I haven't seen any mention of this, sofar.
Is it important to measure stomatal size exclusively on open (or closed) stomata, and if so, which condition is prefered?
May this website help:
https://nph.onlinelibrary.wiley.com/doi/full/10.1111/nph.12667
Dear @Wim de Winter I have attached four PDFs; hope, these could be useful to you.
Best wishes, AKC
Thank you Mr. Elawa & Mr. Choudhary. To my understanding, the eco-physiological question of stomatal pore size does not translate well to my question re. the taxonomical relation between taxon (ploidy) and stomatal size.
In the former, the area and with that, the conductance of the stoma is investigated, which can be estimated indeed from microscopic imagery. Also it studies the relation between environmental conditions and pore size.
In my question, the essence of the matter seems to be standardization of the environmental conditions under which to measure stomatal size.
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