Someone works with analysis of human oral microbiota?

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Would you recommend buying the Maxygene II thermal cycler from Axygen?

We would like to buy a thermal cycler. One of our options is the Maxygene II frm Axygen. Does anyone have experience with this equipment? Would you recommend buying it? Thanks!

08 August 2021 3,453 0 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,211 1 View

How can I calculate plasmid size in nanometers?

Hi everybody, I need to encapsulate a plasmid into small vesicles (50-300 nm) so I want to know how can I estimate a plasmid size (like 7 kb) in nanometers, at least his diameter in its...

11 January 2019 4,676 3 View

Why my PCR is not working anymore?

Few weeks ago I was having troubles with my PCRs, sometimes they work, sometimes don't, so I performed a control PCR in order to check if everything was ok with my conditions and reagents. As a...

09 October 2018 2,986 13 View

Endocrine disruptors in drinking water.

Endocrine disruptors in drinking water. It is possible to assess using bioindicators. What would they be?

02 August 2017 1,061 4 View

Who is working in gingival plastic surgery?

Gingival Plastic Surgery

28 June 2017 8,040 1 View

How to estimate the number of cfu in microbiological examination of air?

I realize microbiological analysis of the air in a semi enclosed environment, by spontaneous sedimentation. The count on the Petri dishs was the 500-1000 ufc. How is the calculation to estimate...

20 June 2017 4,703 9 View

How does occur the seed dispersal of Citrullus colocynthis in natural environments?

I have tried to find out in the literature how C. colocynthis disperses its seeds but it does not seem a popular topic. I found information about seed dormence and germination but the natural...

22 November 2016 872 15 View

Is there any morpho-physiological trait which helps desert plants to deal concomitantly with low water availability and high temperatures?

In desert areas drought often coincides with elevated temperatures. I have worked on cuticular transpiration of desert plants and most of the plant species possess stomata on both adaxial and...

02 November 2016 3,708 7 View

Can anyone recommend software that audio-records and also transcribes?

I have a Mac laptop. Transcription doesn't have to be perfect of course, but to break the back of the work. Thank you in advance.

11 September 2016 2,903 6 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

What role does the gut microbiome play in immune system modulation and disease susceptibility?

Only Probiotics Related

22 July 2024 6,454 0 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

19 July 2024 8,522 0 View

How to extract binding energy from pv.maegz file without using Schrodinger?

I have conducted virtual screening using Schrödinger on a database of 17,000 molecules. Unfortunately, I cannot use the system with the Schrödinger license at the moment. I am trying to find a way...

18 July 2024 2,881 4 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

What is the suitable solvent of MIL-100 for chemotherapy?

I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...

15 July 2024 2,414 1 View