I'm planning to make some Barr's Medium to try to isolate sulphur bacteria but the recipe I have doesn't specify which isomer to use. Could you help me with this?
Dear Mario, it seems that there are 2 enzymatic systems for dehydrogenation of D L lactate. In addition many bacteria may have also lactate racemase that inter-converts these forms of lactate. Therefore, you might follow the advice of Niamah!