I search a protocol to extract Dna and rna at the same time from skeletal muscle. There are some kit like qiagen allprep but it seem that it doesn't work with skeletal muscle. Anyone have tried a kit for skeletal muscle or a specific protocol?
Quentin Chartreux
@Yi Wan no it's not this type of kit that i search, it too simplist and extract Dna and RNA but without separation of rna and Dna.
Maybe the qiagen kit allprep DNA/RNA/miRNA universal can be use because it use proteinase k, anyone have tested this kit on skeletal muscle ?
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Quentin Chartreux
@Utsa Roy i use trizol for rna extraction, but for archived skeletal muscle the Dna extracted with Trizol have a very bad quality
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Quentin Chartreux
Yi Wan
No it's the problem, my sample is really precious that's why i can use my classical protocol (Trizol for RNA, and special kit for DNA)
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