What are reaction conditions for aromatic sulfonation of a dibenzyl ether derivative?

I am using chlorosulfonic acid in chloroform (inert, 2 hr, RT) to para-sulfonate benzyl groups on a dibenzyl ether. The work up is extraction with water. The reaction, however, does not seem to...

04 August 2020 4,446 7 View

Steglich Esterification with EDC/DMAP in CH2Cl2 Extraction - How do I remove excess EDC/DMAP?

I am running a Steglich Esterification with EDC/DMAP in CH2Cl2 on a with the carboxylic acid being a potassium salt that is p-sulfonated. Will the sulfonate group be effected by this reaction? I...

25 February 2020 4,799 3 View

What is the most appropriate emsemble to use for simple AA membrane simulations?

I am working on some more complex membrane simulations and wanted to know if there is a specific advantage to using a certain ensemble. It seems as though most people have used either NVE, NVT, or...

20 November 2019 1,307 3 View

What might I have done wrong with the Comet Assay?

I have just carried out the Comet Assay for the first time. I am using cells are from the MCF10A lineage. I used low melting agarose as well as precoated specially prepared slides for the Comet...

05 April 2016 7,344 6 View

For users of large data sets, how does the establishment of a users' network enhance the user, understanding and accuracy of the data?

I'm working on a piece of research for this - the data set is in the area of inpatient activity (hospitals). I would value your thoughts/ideas on this.

15 May 2013 1,195 10 View

What can explain a low expected heterozygosity rate for T. monococcum wild samples (0.09) ?

I conducted an SDS PAGE electrophoresis on wheat wild varieties to separate their glutenins and measure the genetic diversity of my sample. I only ran one electrophoresis. Can a genetic drift...

14 February 2021 3,971 1 View

Which problems we can find with the acquisition of a foreign language?

It is known that Spain shows a low level of use of English. But why?

01 December 2020 1,177 4 View

QGIS - raster for Minho River and Iberian Peninsula?

Hello! I'm looking for raster files to create a map of the Iberian Peninsula and also the Minho River, in Portugal. Can you recommend me any websites where I can found those? The raster files I...

08 November 2020 1,561 2 View

Can the math teacher in the 21st century work without technology with the Covid-19s preparing to mutate?

In a teaching world, which is becoming unpredictable right now, how can we use technology without losing our teaching capacity? what in Spain is called not falling into technocracies and being...

25 May 2020 4,481 18 View

Tools for measuring patient experience in real practice?

I am interested in different tools that have been implemented in real practice for measuring patient experience (not patient satisfaction), for instance in Spain a questionnaire called IEXPAC is...

02 December 2019 6,609 8 View

Lamp (bulb) for iCycler iA iQ5 bio-rad..?

Hello everyone, Has anyone ever change the bulb (lamp) in a iCycler iA iQ5 bio-rad? Bio-rad does not sell this lamp anymore, and I'm searching for an alternative solution. However a bulb with...

27 November 2019 2,467 1 View

Qsea package - R bioconductor (throubleshooting)?

I have recently started to work with the "qsea" package in r for differential methylation analysis between two groups with data derived from MeDIP-seq. I input my bam files and created a...

07 August 2019 3,348 0 View

Microbiological culture of foraminifers?

Hello , I am looking for a seller / supply source, that can sell me a microbiological culture of living foraminifers. It doesn't really matter what species. Destination: Germany or Spain.

06 July 2019 7,955 2 View

Does anybody know the name of this plant from the Canary Islands?

15 April 2019 1,244 6 View

What do you think about the results of the Spanish election?

Now that election results in Spain are known, do you think there will be a change in Spanish policy, and what will be the effect on European countries and the rest of the world.

03 April 2019 1,017 3 View