Hello I have prepared acidic glycine and basic trisbase for an immunoprecipitation experiment and was wondering if the solutions can be reused in future immunoprecipitation experiments I want to perform?
I've stored these solutions for many months in the fridge. Even the lab would be okay for a few weeks I suspect.
To successfully form an immune complex in an immunoprecipitation, is it better to incubate the protein lysate (that has the antigen of interest) with the antibody overnight followed by the...
16 April 2022 4,775 6 View
i have 2 solvents that i can make use of, they are 1) N,N Dimethyl Formamide 2) Acetonitrile Is there any reagent that i can make us of for the fibres to dissolve in these solvents
28 June 2021 1,937 0 View
I am in search for a good technique for mounting organoid structures for immunofluorescent staining while preserving their morphology. I have tried to use double sided tape to create a...
30 July 2017 6,215 5 View
Can somebody help on how best I can use thematic analysis for data analysis. Will be happy with some literature in this area. Please help!
16 July 2017 3,932 10 View
My research involves assessing banking customers' perceptions on the use of social media by banks. So what would be a reasonable sample in this case since I can not readily quantify how many...
11 July 2017 1,008 12 View
11 official languages but English and Afrrikaans are privileged. There is a visible divide between rural and urban schools in terms of resources. Capable language teachers shun rural...
16 November 2015 5,775 2 View
Post colonial theory
09 June 2014 2,655 4 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hello, I suspect that a molecule I am using is causing bacteria to experience osmotic shock. What chemical properties should I look for to compare this capability with those of other substances?...
06 August 2024 977 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
We are working on biopolymeric hydrogels. Our system is highly viscous and sticky, and the gel formed are high in strength. We are unable to use pH electrode and pH strip. Please suggest an easy...
30 July 2024 942 2 View
Hi, researchers, Is it possible to use a safer acid instead of monochloroacetic acid to convert bacterial cellulose to carboxymethyl cellulose?
29 July 2024 4,570 0 View
Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.
29 July 2024 4,374 1 View
I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.
29 July 2024 8,560 0 View
I am currently performing cell-free DNA (cfDNA) extraction from plasma using an in-house developed kit that employs silica-coated magnetic beads. My protocol works effectively with plasma obtained...
28 July 2024 3,559 0 View
I have been looking for the principle of Cysteine-carbazole-sulfuric acid reaction in research papers and books, but couldn't find it. This reaction is mainly used for the quantification of...
23 July 2024 3,891 0 View