PEG coated Fe3O4 nanoparticles in aqueous NH3?

Hello, I synthesized PEG coated Fe3O4 nanoparticles by coprecipitation methods and I would like to ask whether they can be dispersed in aqueous NH3 since they have a PEG coating and theoretically...

06 February 2021 5,217 4 View

Is there such a function? Is it unique?

21 January 2021 8,962 13 View

What are the current developments in terms research and publications in anthropology in general and social anthropology in particular?

I am interested in examples of anthropological methods that have been applied across different disciplines, for exmple in mathematics education.

20 January 2021 3,090 3 View

How did the tests or exams get to Latin America, specifically the written tests?

I am looking for some bibliographic references that explain how tests or exams (measurement and assessment in education) came to Latin America, specifically written tests. I would like to know if...

13 January 2021 8,607 0 View

Is this true for all values c > 0?

25 December 2020 3,621 45 View

Can children from 1 year old to 15 years be given MODANA and Pfizer Anti-COVID-19 vaccine?

The clinical disease of COVID-19 has been low in children generally and vaccine has been develop.Any evidence of its safety,efficacy and risk for allergy in children? any exemption for children...

23 December 2020 7,177 12 View

Can large samples be stored at -20 degrees prior to RNA extraction?

As part of a Covid project we are sampling urban rodents and performing RNA extractions and serology work. We have too many samples to store at -80 and have opted to perform extractions the same...

15 December 2020 5,252 2 View

Substrate functionalization and antibody deposition for biosensor work?

Hello, I am a bio student working in a lab on his free time and I just started working with an engineering group on sensors. My questions are: 1)Functionalization steps and what are the best...

21 November 2020 7,996 2 View

Why am I seeing RED fluorescence in control non transgenic plants?

Hi experts, I have cloned the DsRED gene into a plant vector under 35S promoter and used it for direct seed transformation of Chenopodium quinoa. When I checked the potential transformed...

24 October 2020 4,775 3 View

Do you think there are any prospects for hydrogen as an alternative to natural gas when operating a gas turbine?

At the moment it is very expensive, the price of 1 kg of hydrogen is from $ 2-5. Are there any prospects for green hydrogen? or will we extract hydrogen from gas? what promising technologies can...

19 October 2020 1,954 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View