Re: What would cause an accumulation of fluorophore in extracellular space?

Hi there, I have setup a GAD2-cre mouse crossed with a Ai32 mouse (containing a Chr2-YFP) in attempt to get channelrhodopsin into the entire interneuron population. After slicing the the mouse...

15 June 2019 1,784 5 View

Statistician wanted for collaboration?

Statistician required for collaboration on a research project. The project sought the opinions of medical imaging professionals about providing a preliminary image evaluation to referrers....

01 May 2019 9,048 5 View

How does one compute confidence intervals for the difference between two binomial proportions in SPSS?

I have a relatively small data set of 135 subjects. These are categorized in two dichotomous variable, type of health care and sex. I can use a Fisher's Exact test to determine if sex is...

05 March 2019 3,847 5 View

Schema therapy , Personality disorders , Older adults . What more can we learn from older patients of PD BPD to enhance therapy ?

I am currently in early stages of qualitative research design in the areas of perceptions and exploration of older adults . I am looking at investigating older adults with personality disorders...

19 February 2019 7,117 5 View

Non-parametric tests: PermANOVAs, or Kruskal-Wallis test?

Hello, Because my data has non-normal distributions, I understand I need to run non-parametric tests. My data includes multiple samples from several distant collection sites. Under what...

22 January 2019 5,467 5 View

Funding is a major problem for me. Is it less costly to check for pathogens in ticks via PCR or antigen/antibody match?

I am unclear if antibody tests can be conclusive in a tick or even if it is possible to test. If so, what are the limitations once the ticks have been collected. Limits such as temporal limits,...

08 January 2019 1,642 7 View

What evalues are recommended for BLASTing fungal sequences?

Hello, I'm using the command line to NCBI BLAST fungal DNA sequences (Table-C1, https://www.ncbi.nlm.nih.gov/books/NBK279684/). The default evalue is 10.0, but one paper I'm following used...

17 November 2018 1,031 3 View

Bacteriophage CE6 wanted - can anyone help?

I have been trying to get a sample of bacteriophage CE6 (which expresses T7 polyemerase) for protein expression studies. The lysogenic form of this is the (DE3) you see in genotypes of cells used...

14 November 2018 2,954 3 View

Grouping read IDs by sample names, and sorting samples into separate fasta files?

Dear colleagues, I pooled together dozens of samples into one library, and sequenced them together. This yielded millions of reads, with the sequencer assigning each unique read-IDs. While the...

08 October 2018 5,513 4 View

What are acceptable thresholds when using BLASTn on fungal DNA sequences?

Hello, I'd like to know your opinions on identifying fungal DNA sequences using the BLASTn with regards to Max Score, Total Score, Query Cover, E-value, and Identity match %? What are common...

06 July 2018 9,896 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View