Hi there, anybody avare of eubacterial primers for real-time pcr, targeting ubiquitous housekeeping gene ? I not looking for 16S targeting primers. I need something elese, some protein coding gene. Thanks a lot for suggestions.
Elhussein Mourad: I want measure (and compare) expression of certain genes in environments with different (and unknown) microbial composition and thus I do not want the results to be affected by 16S copy numbers. If I use protein coding housekeeping gene as a reference, I can roughly assume that there is usually 1copy per each genome. And I also need stable and roughly equal expression in all envionments.