I would like to modify the Protean chamber manufactured by Bio-Rad to allow additional cooling function. This would allow the gel rig to be used for DGGE analysis. I am looking for step by step instructions on how to complete this modification.
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My name is Carl GA Jacob. I am an Auxiliary Professor in the School of Nursing at the University of Ottawa. I am the author of the 2012 research titled: The Use of Experiential Learning in the...
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Hi, im looking at PAL activity in blueberry samples. I use L-Phenylalanine as a substrate and absorbance at 290nm. The absorbance values of my blanks are higher than 1, even my milli pore water...
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existing community-driven development projects in the Philippines
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Where in my dissertation should I explain how I dealt with missing data - Chapter 3 or 4?
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This question was asked in order to know if there are any possible suggestions that we can do to increase the efficiency and reliability of microbiology testing in our country.
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24 October 2023 8,520 3 View
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There are many common mistakes that occur in pre - analytical and post analytical phases that cause erroneous result. I want to know if there's other new research or updated research regarding...
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I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
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Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
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kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
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Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...
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I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?
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