Hello,
I recently followed a sample prep protocol for MS/MS where i eluted my peptide form a mixed mode SCX-C18 cartridge with ammonium acetate (up to 2 M). However, when i tried to dry the sample using speed vacuum, the salt took very long to be eliminated (after 16 hours there is still a big pellet in the eppendorf), in some samples i did not see even much change after most liquid is gone. From literature, NH4OAc should be a volatile salt that can be removed by speedvac.
Did anybody have had the same problem and know how to solve it
Your sample may contains non-volatile salt. Test this by evaporating 1 mL of ammonium acetate solution and compare with 1 mL of your sample in ammonium acetate.
your sample contains other salts, you need to dialyze the sample .
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