hi.i need to have some informations about crypreservation of humans oocytes if it's possible.
Hello Aicha,
The Cryotop method is stated to be a highly efficient vitrification ( flash-freezing process) method for cryopreservation of human oocytes and embryos. Vitrification is much faster but requires higher concentrations of cryoprotectants to be added. The result of vitrification is a solid glass-like cell, free of ice crystals. There are also other methods, the eggs can be frozen using either a controlled-rate or slow-cooling method.
Regards,
hello Dr. Preetha Anil
thank you so much for ur comment
but my real problem in my project, it's that i can't decide until now what's stage of oocyte which i must to choose it for my experience in cryopreservetion ( VG stage or other) and i know that a vitrification as technique is better than normal slow freezing but there are many factors that i must to control them to have a better results as concentration of cryprotectant ( DMSO) and glycerol and the speed of freezing and thawing suitable and temperature...
but my real problem is how can i evaluat the quality of my futur results during freezing and after thawing even after FIV with ICSI and until to have embryos???
and must i to do FISH as technique to see if my oocytes are normals before cryopreservation???
Sorry Aicha I don't have answers to your queries, this is not my area of study, I hope somebody working in this area will guide you.
Post your query in other related groups also.
Regards and Wishes,
Aicha, I have no idea about your project, I wish you all the best and hope by hit and trial you will succeed. ud sharma.
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