Procedure for extraction of components from plants: Take 100 grams of plant and extracted with methanol (500 ml) in soxhlet apparatus for a period of 24 hrs. Then filtere the extarct and concentrated under vacuum results into semisolid mass with respect to dried powder plant crude . Store crude extract st temp maintained at 4º in refrigerator.
Organic component isolation from stored extract: Methanol extract gets purified by column chromatography (silica gel 60-120 mesh) and eluted with step-wise gradient of petroleum ether: ethyl acetate (100:0, 95:5, 90:10, 85:15 ratio) and get analysed by TLC.
The fractions 7 to 10 (100% pure petroleum ether) exhibited similar TLC pattern and combined to provide a pure compound (mg).