Hi all,
I've been attempting to get some action potentials out of my MEA setup for organotypic cortical slice cultures but have had little success so far. Long story short is that we follow the preparation described in Stoppini (1991), and have narrowed the problem down to something biological (the electronics/MEA system are working).
Basically we are running into the problem that we record many neurons firing if we apply various amounts of Picrotoxin - otherwise the recording is completely quiet. Just wondering if anyone has run into similar issues or has some ideas on where we should specifically be looking to troubleshoot.
Thanks!
Carbachol will give you EEG oscillations and if you add 5 mM of reduced GHS, your cells will be gratefull to you.
For more info erad the section on acetylcholine in my review (it is my research section), relevant literature there.
Josh,
if you ask a question and somebody answers, it is polite to acknowledge the answer, ask you mother about polite behavior
Frankly speaking, I dont have any experience in MEA recording. But let me clarify. Do you apply any stimulation protocol except for picrotoxin?
I mean most of neurons in slice/culture won't fire untill they are not stimulated, cause their main afferent paths have been interrupted during prep.
Although in some places you can catch pacemaker neurons activity (see 10.3389/neuro.01.1.1.009.2007 for further info). But most of neurons in slice/culture would make one single spontaneous spike in 5-10 minutes without any stimulation (eather chemical or electrical). Most of chemical stimulation protocols (low Mg2+, high K+, 4-AP, picrotoxin, etc.) are widely used as ex vivo epilepsy models. (see 10.3791/57548).
Hope this will help. Good luck!
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