Dear researcher,
I am currently studying the kinetic of of soil urease with and without the urease inhibitor. Trying to figure out the Km and Vmax values. However, when plotting my data for lineweaver bulk , I got negative intercept. have repeated the assay few times, but still same results.
Probably there might be an error on how I plot the data and there is a better way to do it. Hope u can share it
The way I plotted was using excel and extend the trend line.
Also attached the chart that I plotted and raw data.
Thank you
Dear Nurliana,
If you can provide the original untransformed data, it would be much easier to assist you. There are certainly better ways than the Lineweaver-Burk plot, but tell the best method.
Cheers,
Veli-Matti
Sorry for the prematurely terminated answer. The last sentence should read: There are certainly better ways than the Lineweaver-Burk plot, but to tell the best method original data is required.
Veli-Matti Taavitsainen i have upload the data. Is it because it doesnt follow the michelian? If you could suggest any readings or paper, really appreciate it. tq
Dear Nurliana,
According to your data, it is obvious that Michaelis-Menten kinetics isn't an appropriate model to describe it. The equation for Michaelis-Menten kinetics has the form y = dx/dt = ax/(b+x), but your data suggests rather dx/dt = ax^2, i.e. second order kinetics. Any good textbook about fitting basic kinetic models will give you the information needed. The best fitting methods would require the knowledge of the time points of the measurements, i.e. the data in the form where the measurement times are added into to the table. If you'll provide this information, I can show you how to do the fitting in Excel.
Cheers,
Veli-Matti
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