Thanks alejandro, another consideration is they have to have not been genetically mapped yet (dont want to reproduce existing data) so Id have to see what genome data exists for Diptera, but its definitely worth looking.

We have done experiment with exsitu congervation of Scorpion through laboratory breeding. female scorpion gives birth to 30- 33 young ones at time ,as it is vivparus. All youngs are Identical. which will help to gene mapping. to breed scorpion in laboratory require keen observation. but we did it very easily. scorpions are the living fossils, hence evolutionary point of view it is very important animal. Dr. Vidhin Kamble (India)

We have done experiment with exsitu congervation of Scorpion through laboratory breeding. female scorpion gives birth to 30- 33 young ones at time ,as it is vivparus. All youngs are Identical. which will help to gene mapping. to breed scorpion in laboratory require keen observation. but we did it very easily. scorpions are the living fossils, hence evolutionary point of view it is very important animal. Dr. Vidhin Kamble (India) see Photograph below

There are some other questions to be asked before jumping in, such as how short a generation time should they have (like is one breeding event all you want, or multiple breedings in a year), do you want them to have small genomes (in which case how do you work that out if not studied for genome size), and how inbred do you want the organisms to be? (accepting natural hetrozygosity also varies hugely). Perhaps the best option is to follow on from already funded genome projects, some of which thankfully are starting to look at phylogenetically diverse organisms. Sorry to Alejandro, but there are so many genome-related projects on 'higher' Insects like Flies going on already, please can we devote some money and effort to the other neglected groups? (which i think you want to do looking across the animal kingdom). For taxa, see for example this pdf, on several arthropods and allies that have genomes in progress, and mapping studies can be built on those. Just please stay away from insects, nematodes and vertebrates, there is a vast wealth of data on those already! I'd love it if there we more on the other arthropods like the currently neglected arachnids, and other non-arthropod Ecdysozoan phyla. Pathenogenetic scorpions are actually quite a good suggestion for a 'next-generation model organism'. http://www.genome.gov/Pages/Research/Sequencing/SeqProposals/EcdysozoaProposalFinalPDF.pdf

To address your question sturat, we only need one breeding event, genome size doesnt matter, and our methods are inexpensive enough where we dont necessarily have to use only organisms under funded projects. we are not attempting to build high quality genome assemblies, we are mainly interested in genetic mapping at a resolution good enough to answer our question - whether ancestral organization of genome arrangement is conserved or lost. while we are also interested in neglected groups, for our purposes mapping data is conspicuously absent from most animals, except for those with well assembled genomes. so many organisms who have sequencing efforts still have no usable mapping data because their genome assemblies are still in early stage, or not high resolution enough. while there is a wealth of data on insects already, there isnt too much as far as mapping, and the amazing diversity of insects makes mapping of many insect species non-redundant - because unlike other groups - the conservation status of genome arrangment is not necessarily the same throughout and very unpredictable within the group. we are also looking at other arthropod groups as well have interest in non-arthropod edysozoan phyla - our limitation is what can be bred and provide a suitable # of offspring.

Hopefully, you are aware that most fishes do not eject their nuclei from red blood cells, and thus genetic analysis can be conducted by merely tapping an artery (usually under the caudal peduncle). You don't even have to await reproduction. There any particular reason you need fertilized eggs?

Thanks. We are actually not doing this for vertebrates for several reasons. We need fertilized eggs (embryos, larvae, or juveniles actually) because we are doing genetic mapping which involves analysing the recombination frequency of chosen markers.

I see. Are you interested in Nematostella vectensis (a dwarf sea anemone)? John Baldwin, Florida Atlantic-Davie, may have cultures. They spawn ~100 eggs about every eight days and are easy to culture.

Additionally, when you choose to do vertebrates, supposedly the boreal toad from Wyoming has a furious crossing-over rate. It's supposedly how they can maintain genetic diversity within a relatively tiny population.

we actually did a cross of NV already, and are doing some low resolution mapping - however, the genome assembly of NV is quite good so we wouldnt be adding new data. We arent doing vertebrates right now because we feel that conservation of genome arrangement is ubiquitous in vertebrates (although in chordates it is lost in some tunicates which is interesting) - although it might be a good idea to test some that fit our criteria if their groups isnt well represented. Ill look into the toad and see what the consensus is for amphibians at the moment, thanks.

Sir,

There are many animals that can be used for your purpose such as small insect pests which can be cultured in the laboratory. I would quote a few as Corcyra cephalonica, Tribolium, sitophilus and so on. These insects need special attention of researchers and a solution to get rid of these pests witout a chemical pesticide would be a boon to present day situation. Rafeek.

I have reptilian models you can use. Some have output clutches from 20-75 per lay. However, at oviposition, embryos are already at limb bud stage. But, I would be interested in a collaboration as I have started working on several reptile genomes already.

I have a colony of reproducing hisssing cockroaches. Paternaty is easy to control. They give live birth to 20-30 individuals. Let me know what you think.