Has anybody ever worked with NALF strips to verify PCR products in an end point PCR? What are your experiences? Are the results reliable?
How can we have both environmental and indigenous people protection together with shipping all year-round, as well as natural resources exploitation?
18 July 2024 1,077 3 View
In nanocrystalline metals, the size of their crystalline grains affects how strong, ductile, and hard they are. Smaller grains often mean stronger metals but can reduce ductility. Controlling...
28 June 2024 6,925 0 View
I am working on a 21 sample size data time series, and after applying ADF test one of my variables are integrated of order 2 I(2), two variables are integrated of order 1 I(1) and two are...
08 June 2024 5,787 12 View
Hello, I tried to generate a ligand topology from .mol2 to .str file from cgenff for my molecular dynamics run. The ligand was the product of my molecular docking. However, cgenff gave me this...
27 May 2024 6,483 0 View
I'm trying to remove acrylamide and lactide from the medium that didn't react to form the macromonomer
29 April 2024 4,903 1 View
Cultivating a Love for Reading Amongst 21st Century Learners through Think, Pair and Share.
23 April 2024 4,916 0 View
Use of a maltodextrin-based feed with a lysozyme product to ... Kindly add this article to my profile. Thank you very much Regina
16 April 2024 9,905 0 View
According to the Big Bang theory, our universe began with a singularity, where spatiotemporal curvature is infinite. That means it would take infinite time to come to the present. That's...
19 March 2024 9,482 2 View
Hello, I am currently doing a research about essential oil extracted using soxhlet extraction from guava (Psidium guajava L.) and my solvent is n-hexane. I am just wondering what should be the...
12 March 2024 4,525 4 View
I conducted an XRF test for my research and have a raw data file. However, I noticed that some of the chemical compounds such as K2O, Na2O, Fe2O3, etc. are missing, while their elemental...
02 March 2024 2,756 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View