NALF (nucleic acid lateral flow) strips to verify PCR results?

30 August 2013 0 3K Report

Has anybody ever worked with NALF strips to verify PCR products in an end point PCR? What are your experiences? Are the results reliable?

More Regina Mark's questions See All

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Infinite order of Laguerre?

If Lmn(x) is the Laguerre polynomial of order m and degree n Is there a means of computing Lmn(x) for 1) m goes to infinity 2) n goes to infinity

08 February 2021 6,320 4 View

Sharpening algorithm for similar images with different levels of blurring?

Say for arguments sake I have two or more images with different degrees of blurring. Is there an algorithm that can (faithfully) reconstruct the underlying image based in the blurred...

17 January 2021 7,324 3 View

How to make a pushover Analysis of a reinforced cantilever beam in ABAQUS?

Hello everyone, Im doing a pushover Analysis of a cantilever beam in ABAQUS, made of reinforced concrete, but my model doesnt seem to converge proberly. The program simply aborts before hitting a...

15 January 2021 1,627 3 View

How to illustrate FE Panel Regression with LSDV in a research framework?

First time to use FE Panel Data Regression using LSDV, and not familiar with how to illustrate it in a research framework. We have regional CSR score as IV and Social Development ( four areas:...

14 January 2021 580 3 View

Domestic ancient Egyptian gods and goddesses?

Who are the gods that ancient Egyptians thought of as closer to them and could have a personal connection with them?

25 December 2020 5,505 1 View

Could someone help me recheck my identification of my moth samples?

Greetings to all! I am humbly asking for some help, in regards to the checking of my moth samples. Though it is of great inconvenience to you all, I will respect your decision nevertheless....

08 December 2020 6,009 5 View

Is there a relationship that can be used to relate flow rate (m3/s) to MPa in fluid injection rate?

I am doing simulation exercise of which I have to inject fluid pressure into a borehole. The parameters I am using the injection rate is given in pressure (MPa) and the software accept injection...

01 December 2020 3,221 2 View

What is the current and voltage profile for a short dipole antenna when the monopoles have different lengths?

The textbooks show a triangular pattern for the current as the limiting case for a short dipole, and how this may be considered to be the limiting case for the sinusoidal current distribution....

26 November 2020 5,280 5 View

Best way to teach Data Structures and Algorithms?

Next semester I am teaching an online class on Data Structures and Algorithms. The students will have had two programming classes in C++ and Java each. I am looking for a way to engage the...

11 November 2020 1,176 4 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View