I am using a UV/VIS detector mobile phase 0.1 FA and ultra-pure water, what could be the problem here? only the solvent peak is visible.
Check the instrument settings: Verify that the instrument parameters, such as the flow rate, gradient, detector wavelength, and temperature are set correctly for the analysis.
Clean the autosampler
Check the T. TUBES for further detection. Is it possible to note the type of sample you are using on it. Change parameters and re-run.
I knew it's very basic but most of the times it works
My column is fairly new and clean and operates well in other HPLC instruments, my method is a gradient elution method, and my solvents are ACN and water. I have removed the column and set a union...
13 March 2023 1,078 4 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!
11 August 2024 3,770 4 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
A website software of Blackbody radiation law expert software can used through the following web site. http://39.105.188.151:3000/index
07 August 2024 1,706 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View