Zeta potential is most commonly measured by light scattering.

Today, half a dozen companies make such instruments including Malvern Panalytical, Brookhaven Instruments, and Wyatt Technology).

The technique has evolved into a mature platform and is often used to measure dispersions in high concentration aqueous salt solutions. High typically means 10mM or so.

To achieve this, instruments rely on phase analysis light scattering (PALS) that I invented in the late 1980s.

But there is an increasing need to measure under even higher salt concentrations, namely physiological fluids such as PBS X1.

Based on many questions on RG - and their answers - such measurements are proving difficult to make and interpret.

So, for the discussion point :)

Do you measure samples in physiological fluids (such as PBS X1 buffer)?

What are your experiences?

How important is measuring zeta potential for your application?

What kinds of things are you measuring? And why?

Do you know why it is so much harder to make the measurements?

I'm very interested to learn about people's experiences - good and bad.

Thanks.