I am trying to understand the functioning of an UV Mini 1240 Shimadzu but I do not understand my data. I might use the wrong set up because what I see on gel when I extract DNA is correct but the concentration and 260/280 ratio I see on the machine is not matching. Does anyone knows how to calibrate such a machine? Before I was always using NanoDrop technology, which is a one-button function. Is it possible to calibrate the machine and make it work? I see on the manual that the value of A260/A280 is related to a constant (K) but is not indicated for DNA measurement and I think it might change if measuring protein or wavelight.
If someone has some info about it, please contact me.
http://www.phywe.fr/index.php/fuseaction/download/lrn_file/bedanl.pdf/35655.93/e/3565593e.pdf
Thanks in advance.