Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

Is there any guide to determine sufficient sample size in relative important index (RII) analysis ?

Relative important index (RII), is often used in determine the ranking of measured items in quantitative data. Based on my search from literature, the sample sizes range from 32 to 150. So, is...

19 November 2017 2,150 3 View

Can I use Ammonium Bicarbonate as buffer if I dont have TEAB for iTRAQ experiment?

I came to know that Tris having primary amine group may interact with iTRAQ labels, and TEAB is an advised buffer to be used post precipitation; but if I don't have TEAB at present can I use...

24 November 2016 3,478 3 View

Does anyone have any idea about the reverse protein hits generated by Protein Pilot?

I am facing one problem with identification carried out using Protein Pilot, I get many hits whose description shows reverse protein. Does anyone have any explanation for this? Use of Mascot...

17 September 2016 138 6 View

What does zero peptide mean in protein pilot data analysis?

In Proteomics, while doing analysis on protein pilot after running my sample on mass spec; I got plenty of protein IDs which are having zero peptide identification to the library. I don't...

14 January 2016 4,987 3 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

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02 March 2021 3,060 3 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

Does qualitative research using closed and open ended survey and semi structured interviews qualify as triangulation?

I am using qualitative research to look at employee motivation. I will use a closed and open ended survey (for biodata information and general views to motivation) and semi structured interviews...

18 February 2021 436 11 View

Should I include less important items in con-joint survey experiment?

I plan to do a conjoint survey experiment in which I randomly change attributes of hypothetical job candidates and show two types of potential candidates with different attributes. Then, I ask...

16 February 2021 5,812 1 View

How can we measure the reliability and validity of our survey questionnaire?

We are conducting survey research on COVID-19 misinformation among students. How can we validate our survey for it to produce valid and reliable data? Also, what statistical tools can we use to...

16 February 2021 678 3 View

What would be the best survey design and stat. analysis for my research- impact of pandemic on pro-conservation and pro-environmental behaviours?

Ive started to design the survey with demographics, I have a list of pro-environmental and conservation behaviours which i plan to ask if they have changed since the pandemic. I think i'll use...

15 February 2021 4,204 3 View

Bootstrapping: to use or not to?

Hello, I would need help w.r.t deciding on an approach. I sent out a survey and about 2000 of 15000 recipients completed the survey. Doubts have been raised on accepting the results based on...

15 February 2021 5,585 13 View