Markers for Drosophila enteric nervous system?

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

02 August 2024 9,518 2 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View

How can reinforcement learning algorithms be effectively applied to study decision-making processes in neuroscience?

I am interested in exploring the intersection of neuroscience and AI, particularly through reinforcement learning for understanding decision-making in the brain. I also want to integrate...

17 July 2024 1,225 0 View

What is the difference between neuropsychology and cognitive neuroscience ?

The main differences

14 July 2024 3,158 4 View

Does the temporal summation of EPSPs accumulate linearly to action potential threshold or logarithmically to a plateau/limit?

I am attempting to determine which current inputs (in uA) are subthreshold for inducing a burst of activity. This is because I am attempting to model DBS form epilepsy in vitro, which clinically...

23 June 2024 4,083 1 View

Did you ASK AI (https://talkai.info/tr/chat/) the same question?

What food do intestinal bacteria like most?ChatGPTIntestinal bacteria love fibrous foods that are prebiotic. These foods include vegetables (such as broccoli, cabbage, and brussels sprouts),...

22 June 2024 4,567 0 View

Do plants produce hormones that have the same influence properties of animal hormones?

I know that all animals and insects produces hormones. Do plants produce hormones that can have an equivalent effect on an animal like animal hormones?

13 June 2024 3,860 1 View

Our -20 Freezer thawed unexpectedly, can freeze-thaw of K-glu internal solution cause issues obtaining Gigaohm seals?

We are an electrophysiology lab out of Milwaukee. Recently, due to ice buildup, a small crack in the door of our -20 freezer resulted in all of the freezers contents reaching room temperature...

06 June 2024 2,234 1 View