I have for many years been measuring bacterial production using 3H-Thymidine and 14C-leucine. For these measurements I have used the Scintillation Cocktail “Ultima Gold”, but this cocktail has become rather expensive and I am therefore looking for an alternative.
Does anyone has experience using the “Cytoscint scintillation cocktail”? It is around half the price, but the question is, does it work ok?
Thanks for your help.
Hello Christian,
I never worked with the "new" cocktail indicated by you.
But, If I was which try to undertsand the performance of the new cocktail, I start with some tests in which put in comparison the "old" cocktail with the "new" cocktail.
Use the same ratio sample: cockatil, the same total volume, same vials and same "sample": under these conditions, you could buy ethe cockatails and undertsand if the new cockatail are able to give you the same performance with the old cocktail, and then undertsand if you can perform your experiments with cheapper cost
Mattia
Dear Mattia, thanks for the answer. I will try and test this out. Best wishes Christian
hello Christian,
The cocktail type ultimately depends on the kind of filters you use (in case you use the filtration method you want the filters to be dissolved prior to counting). In all other cases, I think it does not matter what cocktail you use. In our laboratory, we use Filter count or Hionic fluor (for 3-H thymidine, filtration method ) and Ultima Gold for 3-H leucine (microcentrifuge method). I attached Perkin Elmer's recommendations
Thanks Stefanija for your feedback. I will also try and look into this.
Christian
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