Has anyone come across an optimal protocol for the amount of cells and/or concentration of protein per well (96 well) using MAPK Family Activation ELISA Kit?
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
How can we differentiate between calcite, dolomite, siderite, magnesite and ankerite minerals in carbonatite rocks in thin section under optical microscope?
07 August 2024 2,132 3 View
These days policy coordination is becoming necesssary on different front. In India also it is the same case. Monetary and Fiscal policy interface is important in order to address the...
30 July 2024 2,104 6 View
The indexes NDWI and MNDWI are use to identify waterbody. Can I use these index to identify wetland also? Thank you for advance.
25 July 2024 6,242 2 View
Hi there, I did a CFA to 10 items on 2 latent variables. The model fit is too good and I've never seen it before. CFI / TLI = 1, RMSEA = 0. Is it okay, if everything else was done properly?...
24 July 2024 5,454 7 View
Organic Synthesis. My student has been trying iodination of arenes (our goal is to iodinate p-phenylenediamine) following these literature procedures: 1. Synthesis 2004, No. 11, 1869-1873 using...
16 July 2024 6,630 1 View
Hi everybody, I wonder is there a way to prepare foam from iso-propanol/water mixture? The content of this alcohol should be 70 – 80 w.%. I plan to use silicone-based surfactants, I have several...
15 July 2024 3,539 0 View
Your ideas could spark the next big thesis or dissertation for students eager to make an impact in their field.
14 July 2024 9,999 0 View
There exists a neural network model designed to predict a specific output, detailed in a published article. The model comprises 14 inputs, each normalized with minimum and maximum parameters...
14 July 2024 2,714 3 View
Mangroves were reported to eat into limestone atolls like so much cake - but could it have been diatom partnerships at mangrove roots that ate into the rock? If gypsum formation is a clue, how...
12 July 2024 159 2 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have different polymer solutions and I hypothesize that water molecules are retained based on the water-polymer interactions, so the amount of free water is less to intercat with drug tablet....
28 July 2024 9,545 6 View
I am a beginner with ImageJ and would like to know how to quantify fluorescence images of platelets taken with a fluorescence microscope at 40x and 100x magnification. If you could provide...
11 July 2024 1,464 7 View
Hi all, I am working on a project which needs expansion of CD45+ selected cells from tumor and lymph nodes. My Tumor samples expand good but my samples from the lymph nodes are less and less...
23 May 2024 2,839 0 View
I am a bit confused by these statements: "For extracellular stimulations, a cathodic pulse can generate a current sink in the space immediately around the stimulation electrode, thereby...
11 May 2024 3,318 2 View
I am using ImmunoCult™ Human CD3/CD28 T Cell Activator (Semcell, Catalog #10971) to activate PBMC and expand T cell. but the T cell activator contains an anti-CD3 antibody clone that fully or...
07 May 2024 2,973 3 View
How much water is typically used during the impregnation step? is there any relationship between the weight of carbon and the amount of water?
12 April 2024 2,952 0 View
Hi I`m trying to detect immune response in vitro with my nanoparticle (virus-like particles) incorporating SARS-CoV-2 Spike protein. I inoculated quite a lot of nanoparticle in PBMC and inoculated...
09 April 2024 174 2 View
hello everyone i want to activate the 4 successissevement domains 1 then 2 then 3 and 4 on comsol multiphysics after a certain timeout t=10s? what method can i use i tried with activation under...
26 March 2024 6,435 1 View
Hello, what is the best way to find partial pressure order. I am proposing the following and correct me if I am wrong ? Obtain Activation Energy and pre-exponential factor from ( Coat-redfern)...
06 March 2024 1,073 2 View