I am planning to buy 2 Glan Taylor polarizers for my experiment. One for polarizing my laser beam vertically (excitation beam). The other one at 54.7 degrees from the excitation beam polarization. I am detecting fluorescence at 90 degrees from the excitation beam. My question is: does it matter if I use Glan taylor or Glan thompson polarizers? Or more specifically, what type of polarizers should I use for attaining magic angle conditions?
Saurabh,
Glan Thompson prisms differ from Glan Taylor prisms in that the optic axis is is in the plane of the cut and cement is used between the two prisms, which allows for a larger acceptance angle and reduced reflection losses, However, if you are working in the UV be sure the cement won't filter out that part of the spectrum..
Cheers,
John
Thank you John for your answer. My laser works at 440 nm and I am collecting fluorescence (so lambda>440 nm). Therefore, I think, Glan Taylor should work just fine. Moreover, I am planning to buy Glan Taylor with AR coating which would allow more transmission in 400-500 nm range.
Can you please elaborate the need of magic angle in fluorescence lifetime measurements?
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