anybody have a good protocal for plant digestion for icpms. highly apprciated .
Please find enclosed some links and PDFs for reference...
https://crustal.usgs.gov/laboratories/icpms/solution.htm
Multielement Plant Tissue Analysis Using ICP Spectrometry (Plant Mineral Nutrients pp 121-141,Part of the Methods in Molecular Biology book series (MIMB, volume 953))
Abstract : Plant tissue analysis is a valuable tool for evaluating the nutritional status and quality of crops and is widely used for scientific and commercial purposes. The majority of plant analyzes are now performed by techniques based on ICP spectrometry such as inductively coupled plasma—optical emission spectroscopy (ICP-OES) or ICP—mass spectrometry (ICP-MS). These techniques enable fast and accurate measurements of multielement profiles when combined with appropriate methods for sample preparation and digestion. This chapter presents state-of-the-art methods for digestion of plant tissues and subsequent analysis of their multielement composition by ICP spectrometry. Details on upcoming techniques, expected to gain importance within the field of multielement plant tissue analysis over the coming years, are also provided. Finally, attention is given to laser ablation ICP-MS (LA-ICP-MS) for multielement bioimaging of plant tissues. The presentation of the methods covers instructions on all steps from sampling and sample preparation to data interpretation.
Dear Dr. Saman,
Take approx. 800 mg of sample and heat with 10 ml 70% Nitric acid followed by gentle heating with addition of 8 ml perchloric acid until colourless. Let the solution to cool and add 30 mL of dd water and resume heating for 10 min. Finally, cool the solution and make up the volume to 100 mL. Now, solutions are ready for analysis via ICP.
Source: http://www.agilent.com/cs/library/applications/5988-4450EN.pdf
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