Dear Shiva,

first, you increase lipids accessibility by pre-treatment of the dry algae with water and an ionic liquid plus micro waves (for cavitation, i.e. growth and collapse of bubbles) for disruption of cell membranes. This is a preparatory step, but not an extraction step as yet. Next, you extract the lipids from the previously solid, now pre-treated dry algae using a mixture of chloroform and methanol. Therefore, this is a classical solid-lipid extraction process.

Wishing you best of success,

Johannes

Dear Shiva,

I think you can use non polar IL cathion and RTIL, so the lipid can extract directly. Then, you can separate between lipid and IL by precipitation principle, because lipid will be solid phase in room temperature.

Best,

Mega