Protein Quantification Outside Standard Curve?

I am running a Bradford protein assay and my protein values are falling beyond the standard curve. I am already using 1uL of sample in the standard microplate assay. Is there a way to extend the...

13 December 2020 6,672 3 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Cs-Gluconate for intracellular solution?

Dear patch-clamp enthusiasts, For patch-clamp recordings of vomeronasal sensory neurons, I need a cesium-based intracellular solution. Because I also need a defined amount of chloride, I have to...

11 November 2020 6,384 7 View

PLS- Is it normal that the standardized regression coefficients of my model do not coincide with the VIP values?

I am trying to perform a partial least square model (PLS) using one response variable and 33 explanatory.. Before the analysis I have standardized all my data so i am expecting that the regression...

19 October 2020 6,702 2 View

Which is preferable for whole rat brain clearing: tert-butanol or xylene?

I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.

05 October 2020 1,306 1 View

Is recoding negative questions always necessary when downloading to SPSS from SurveyMonkey?

I formatted a survey in SurveyMonkey using questions from a valid and reliable instrument (Principles of Adult Learning, Conti, 1978). Questions used a 6 point Likert scale (Always, Almost...

29 September 2020 8,194 4 View

Clarification on Shapiro-wilk sig. , skewness and kurtosis?

Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...

29 August 2020 1,384 4 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Google Earth Engine for Multitemporal analysis (Land Use Land Cover Change)?

Hello. I am reading about Google Earth Engine (GEE) and their applications for vegetation, landcover and agriculture mapping. I am analyzing an area of 20Mha to determine a multitemporal changes,...

01 May 2020 2,059 11 View

Why do we use qPCR with a singular reference primer?

Hi, I am doing a qPCR and using a reference primer for my lab research project. However, I am not sure what is the purpose of having a singular primer for my reference gene and having both...

28 February 2020 1,636 3 View

Is there any rich, publicly available English literary corpus?

Hi everyone. I am looking for a rich corpus in English literature, a one which includes literary prose ( e.g., novels or short stories) . can anyone propose a good one? Best regards Fatemeh

01 March 2021 1,970 2 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View

Your thoughts on the spherical densiometer to estimate relative species canopy cover?

Hi biologists and biogeomorphologists, I want to characterize vegetation plots on a transect that follows a successionnal gradient from a river bed to a mature forest. Ecologically, I am...

26 February 2021 5,720 2 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View