Actually i tried to get fatty acid profile of maize leaf tissues using GC-MS analysis, but i am unable to get results.
I have incorporated 'heptadecanoic acid' as an internal standard in the extract during preperation, but thats also not visible in the GC-MS chromatogram, only noise appears.
1. Question: is your system working properly? Do other methods work, a quality Control Sample e.g alkane? Watch the Injection.
If everything is fine --> start esterification with your C17:0 alone. Signal? Then add the C17:0 always One step earlier in the protocol and tell us where you lose It.