And please put the complete practical procedure of shoot C/N ratio analysis
bests,
We use Dumas combustion equipment to measure C and N in plants. The concentrations of C are so large that variation is small. I have not seen data at the tissue level for C:N. Why do you want to know?
thanks Mr Milham,
I'm analysing CN ratio to see its relation with number of flowers on blueberry lateral shoots
I need more details about it, if you or anyone else could help
Dear Fraidoon
C,N in the middle and the base of shoots are differ from shoot tip in the ratio and formula.shoot tips have more nitrogen content than rest part of the shoot in contrast of carbohydrate content , furthermore flowering and fruiting depend on carbohydrate reserves in mature part of the shoot which, so that estimation of C:N ratio should be conducted using other parts of the shoot ,not tips.
best regards
Ali Alhayany
.
Dear Ali,
thank you very much for your information, appreciated.
but in Blueberry, the reproductive part is the shoot tip.
is your information about blueberry or any other crop, please?
Hi Fraidoon,
I think your question is very relevant, considering the floral initiation occurs very early in the new growing tips. In fact growth ceases only as floral meristems are initiated (you can check one of my papers on inflorescence bud initiation in blueberries). With this, the tender tissue where flowers are being initiated is likely still partially a sink, rather than source. The Darnell Lab at University of Florida has done some research in carbon partitioning in the plant, but nothing as specific. I added links to some of the publications.
Hope this helps.
Best,
Al
http://journal.ashspublications.org/content/116/5/856.full.pdf+html
http://journal.ashspublications.org/content/121/6/1132.full.pdf+html
http://journal.ashspublications.org/content/129/3/294.full.pdf+html
hello A P Kovaleski,
thank you very much for your answers, they are really helpful. Appreciated.
Yes , there will be distinct difference in C:N ratio of shoot tip to other parts of the same shoot , since shoot tips dont have phloem . Likewise , if you compare the soot tips from different plants , probably , you may not find much differences , but with respects to other shoot parts , such differences will be more conspicuous across different plant species.
Thank you very much Srivastava sir,
Yes, I'm comparing different parts of the same shoot, since that is only the shoot terminal area which produces flowers in blueberry. I'm looking for the reason(s) relevant to C:N balance of it compare to other parts of the shoot.
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