Is there any test for the estimation of total glycosides in a sample?

More Saadia Ijaz's questions See All

Hello can anyone please tell how to convert 24 ug/ml chlorophyll-a concentration into mg/g dry weight extracted from 10 mg dry biomass?

I have extracted 24 ug/ml of chl-a from cyanobacteria from 10 mg freeze-dried biomass in 1ml 90% acetone. How can I convert it into mg/g dry weight?

07 August 2016 1,598 7 View

Can anyone suggest an impact factor journal for chemopreventive activities of phycobiliproteins with very fast reviewing time?

I need to publish an article about phycobiliproteins and their chemopreventive potential. Please suggest an impact factor journal with less review time and high chances of my paper to get publish.

06 July 2016 1,784 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to code open-ended questions in SPSS?

Hello all, In SPSS I am going to code 2 open-ended questions. I have already read all the answers and I made a list of the most important categories to which I can code the answers. This question...

02 March 2021 1,757 4 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

Satellite orbit estimation using Recurrent Neural Network - what should I use?

Hello, I am a master's student studying in Yonsei Univeristy, Korea. I am trying to estimate the state of satellite, using Neural Network. Below is a simple flow of my study. 1. Train (t0 ~ t1)...

23 February 2021 6,383 3 View

Saadia Ijaz

Thank you very much!

Catherine M G C Renard

Good old fashioned Dubois method (phenl sulfuric) for total sugars. Just be careful of the sulfuric acid.

Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350-356.

Thank you so much Catherine!

Toskë L. Kryeziu

These should help you:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1269789/

http://www.jbc.org/content/246/2/430.short

http://onlinelibrary.wiley.com/doi/10.1002/0471142913.faa0101s06/abstract?userIsAuthenticated=false&deniedAccessCustomisedMessage=