Yes, derivation of EC50 and LC50 for diatoms and copepods were studied by you in your project. I surprised how such doubt comes in your mind? Pls refer the report you prepared
The toxicity values for Ni on plankton is required to compare the toxicity of Ni with other groups/genus/species of phytoplankton and zooplankton from Indian coastal waters.
Yes, the LC50 values derived based on the dose-response of toxicity bioassays by exposing organisms to gradually increasing concentrations. We have conducted such toxicity bioassays on eight species of marine organisms from coastal waters of Tamil Nadu, India and derived toxicity values. In order to compare and derive safe limits for Nickel the toxicity values for nickel on marine organisms of Indian coasts is required.