Is there any other enzymes like hyaluronidase that can dissolve stroma components?

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How to validate anti-human-immune-checkpoint in mouse model (in vivo)?

The antibody is against human protein, but with mouse IgG (as it is generated in mouse). Before totally humanizing it (which takes a lot of money and time), I want to validate its function in...

20 April 2022 9,928 0 View

Can FDA-approved drugs be used in other disease setting?

Hi Everyone, I'm exploring the anti-tumor effect of some drugs. I'd like to test some FDA-approved drugs which are not used to treat cancer. My question is, if there is any anti-tumor effect for...

14 December 2020 1,630 1 View

How to do proper human T cell proliferation assay?

Hi Everyone, I did numerous human T cell CFSE proliferation assays, but the peaks never shift. I put CD3/CD28 and IL2, and I can see that T cells did proliferate by my eyes (as there was increase...

08 December 2020 9,238 3 View

How to know if my protein forms a dimer or not?

Hi everyone, The protein I studied is a membrane protein. Some study indicates that there is possibility that this protein can form a dimer. I did standard western blot for this protein and my...

20 October 2020 7,150 7 View

What assay should I do to confirm membrane protein-protein binding?

Hi all, My hypothesis is that CD99 binds to CD88 (two membrane proteins). My colleagues suggested me to do co-IP, but I don't have good western blot antibody against one of the protein. However,...

14 October 2020 9,377 6 View

How to ensure a marker antibody won't activate T cells and affect the result?

I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...

06 October 2019 7,384 3 View

To synthesize chimeric receptor, should I used real gene sequence or artificial sequence?

I'm synthesizing a chimeric antigen receptor (CAR), and I have the whole sequence of the amino acid. Now the problem is, to ask company to synthesize the DNA of the CAR, should I send real gene...

06 February 2019 1,641 1 View

Why my gene in single-cell sequencing showed zero value?

I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...

03 January 2019 1,876 3 View

Why can't I detect the surface protein by western blot?

I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...

29 November 2018 6,457 3 View

For HDAC inhibitors, which HDAC member(1/2/3/4...) is inhibit by it?

There are so many HDAC family member (1-11), but which is the one that is inhibited by HDAC inhibitor?? I ask this question because I found some of them seemed to be tumor suppressor, while some...

25 March 2018 652 2 View

Do you think Serotonin is Primarily A NEUROTRANSMITTER? Does anyone else like me think this Beautiful Biomarker was repurposed as a Neurotransmitter?

Serotonin (5-hydroxytryptamine, 5-HT) is predominantly known as a neurotransmitter in the central nervous system (CNS), where it plays a crucial role in mood regulation, gastrointestinal motility,...

20 July 2024 3,925 5 View

What is the association and mechanism between bacterial diversity in the oral microenvironment and clinical manifestations of periodontal disease?

This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...

09 July 2024 6,210 1 View

How to deal with lab test values above quantifiable limit?

Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...

22 June 2024 6,181 1 View

Primary salivar tumors in bone?

Although rare, some salivary tumours can grow primarily in nearby bones instead of salivary glands. The diagnosis of said tumours is defined by Batsakis criteria (1979). I would like to know if...

10 June 2024 8,967 2 View

How to calculate tumor size estimated doubling time when the initial volume is zero?

I am writing a case report on a rapidly growing symptomatic brain tumor. The patient had an initial brain MRI which was unremarkable, and no brain tumor identified. after 47 days the patient was...

26 April 2024 6,861 0 View

How does the immune system become activated against mRNA and eliminate it?

Hello, I have 2 questions regarding cancer mRNA vaccines. When synthetic mRNA vaccine for cancer is introduced into the body, our expectation is that the immune system will be activated against...

09 March 2024 2,858 2 View

How to do tumor mouse model properly?

Hi guys, I'm working on the tumor mouse model. In general, I prepared tumor cells and injected cells into the flank of mice subcutaneously. After around 3 weeks, I found, in the same group,...

29 February 2024 9,034 3 View

What is the most efficient approach to integrate eXplainable Artificial Intelligence (XAI) techniques into brain tumor detection systems?

I'm doing some research to explore the application of eXplainable Artificial Intelligence (XAI) in the context of brain tumor detection. Specifically, I aim to develop a model that not only...

25 February 2024 2,490 5 View

Is the intraperitoneal injection of a cellular therapy effective in subcutaneous tumor models?

This route was primarily used to treat intraperitoneal cancer models. I was not able to find references for subcutaneous models.

18 December 2023 6,155 3 View

How should I report the objective reponse rate (ORR) ?

My team conducted a prospective objective clinical study where we followed-up cancer patients who took a certain medication while undergoing conventional chemotherapy. We used objective reponse...

14 December 2023 9,517 1 View