In tumor microenvironment, stroma seems to be an important factor that makes tumor immunosupressive. Is there any other enzymes like hyaluronidase that is proved to dissolve stroma components?
In vivo that job is mainly performed by metalloproteses and beta cathepsin.
And do not forget heparanase and uPa.
The role of various types of matrix metalloproteinases are highlighted durin g the process of tumour invasion and also during angiogenesis..
The antibody is against human protein, but with mouse IgG (as it is generated in mouse). Before totally humanizing it (which takes a lot of money and time), I want to validate its function in...
20 April 2022 9,928 0 View
Hi Everyone, I'm exploring the anti-tumor effect of some drugs. I'd like to test some FDA-approved drugs which are not used to treat cancer. My question is, if there is any anti-tumor effect for...
14 December 2020 1,630 1 View
Hi Everyone, I did numerous human T cell CFSE proliferation assays, but the peaks never shift. I put CD3/CD28 and IL2, and I can see that T cells did proliferate by my eyes (as there was increase...
08 December 2020 9,238 3 View
Hi everyone, The protein I studied is a membrane protein. Some study indicates that there is possibility that this protein can form a dimer. I did standard western blot for this protein and my...
20 October 2020 7,150 7 View
Hi all, My hypothesis is that CD99 binds to CD88 (two membrane proteins). My colleagues suggested me to do co-IP, but I don't have good western blot antibody against one of the protein. However,...
14 October 2020 9,377 6 View
I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...
06 October 2019 7,384 3 View
I'm synthesizing a chimeric antigen receptor (CAR), and I have the whole sequence of the amino acid. Now the problem is, to ask company to synthesize the DNA of the CAR, should I send real gene...
06 February 2019 1,641 1 View
I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...
03 January 2019 1,876 3 View
I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...
29 November 2018 6,457 3 View
There are so many HDAC family member (1-11), but which is the one that is inhibited by HDAC inhibitor?? I ask this question because I found some of them seemed to be tumor suppressor, while some...
25 March 2018 652 2 View
Serotonin (5-hydroxytryptamine, 5-HT) is predominantly known as a neurotransmitter in the central nervous system (CNS), where it plays a crucial role in mood regulation, gastrointestinal motility,...
20 July 2024 3,925 5 View
This research question explores the complex relationship between bacterial diversity in the oral microenvironment and the clinical manifestations of periodontal disease. The oral microenvironment...
09 July 2024 6,210 1 View
Hi, I'm analyzing data from an oncology trial and trying to perform statistical analysis including cox regression analysis and paired t-tests. My data includes laboratory test values, including...
22 June 2024 6,181 1 View
Although rare, some salivary tumours can grow primarily in nearby bones instead of salivary glands. The diagnosis of said tumours is defined by Batsakis criteria (1979). I would like to know if...
10 June 2024 8,967 2 View
I am writing a case report on a rapidly growing symptomatic brain tumor. The patient had an initial brain MRI which was unremarkable, and no brain tumor identified. after 47 days the patient was...
26 April 2024 6,861 0 View
Hello, I have 2 questions regarding cancer mRNA vaccines. When synthetic mRNA vaccine for cancer is introduced into the body, our expectation is that the immune system will be activated against...
09 March 2024 2,858 2 View
Hi guys, I'm working on the tumor mouse model. In general, I prepared tumor cells and injected cells into the flank of mice subcutaneously. After around 3 weeks, I found, in the same group,...
29 February 2024 9,034 3 View
I'm doing some research to explore the application of eXplainable Artificial Intelligence (XAI) in the context of brain tumor detection. Specifically, I aim to develop a model that not only...
25 February 2024 2,490 5 View
This route was primarily used to treat intraperitoneal cancer models. I was not able to find references for subcutaneous models.
18 December 2023 6,155 3 View
My team conducted a prospective objective clinical study where we followed-up cancer patients who took a certain medication while undergoing conventional chemotherapy. We used objective reponse...
14 December 2023 9,517 1 View