Is there any methodlogy for sliding door track arragment?

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How to subculture Expi293F cells?

Hello everyone; I am trying to develop HEK293F suspension cells for recombinant protein production. I use the Expi293 Expression System kit for this. HEK293F cells were cultured in Expifectamine...

27 November 2023 9,008 6 View

What do you think is the best method for bacteriocin isolation from lactic acid bacteria?

Hi everyone, I'm working on the isolation of bacteriocin from lactic acid bacteria. I've a bacteria that I isolated and know to be lactobacillus. When I cultivated this bacterium in liquid culture...

04 March 2023 842 18 View

How can I solve my SDS-PAGE problem after ammonium sulphate precipitation?

Hello everyone; After precipitating the proteins with ammonium sulphate, we performed SDS-PAGE. However, there was a problem with the gel running, we couldn't see any tape. We did dialysis to...

13 February 2023 3,872 5 View

Do we need to clear the outliers before Confirmatory Factor Analysis?

Hello everyone, I have a normally distributed data. Before conducting CFA, is it necessary to remove outliers? First, I removed two of the outliers and conducted CFA, my CMIN/df value was...

22 November 2022 6,087 7 View

What is one important difference between the abstract and the conclusion?

In general, it’s important to specify the topic, aim and scope of your research in the abstract. The vast majority of journals select papers for publication just by reading their abstracts. Hence,...

10 December 2021 7,738 4 View

What is the difference between a Scopus journal and an ISI journal?

Some journals are located in the two data bases and some are located in one without the other. So what is the difference and which one is more distinguished or superior to the other?

06 December 2021 9,946 8 View

I am trying to amplify my gene of interest (GOI, 2000 kb long) by PCR. My previously designed primers are not working. How can I optimize the primers?

As I wanted to amplify my GOI, I chose first 18 nucleotides including start codon in my forward primer. I add BAMHI recognition site at the beginning and 3 nucleotide extension for the efficient...

12 January 2021 3,605 3 View

I treated my fixated cells with PI for Cell Cycle Analysis but our Flow Cytometry did not work. How long can I keep my samples stable(+4) with PI dye?

I prepared my samples with ethanol, RNase and PI. However I could not use the Flow Cytometry machine because of an technical problem. I covered my samples with foil and put them in +4. As my...

21 November 2020 9,398 6 View

Would longer ROCKi exposure change iPSC growth metabolism?

I am routinely using the ROCK inhibitor for iPSC passaging. 1 day after seeding, I normally switch to iPSC media without the ROCKi but once I left it over the weekend with ROCKi and surpassingly...

08 January 2020 1,976 4 View

What are new research trends in medical electronics?

The new developments in medical electronics, aims to facilitate significantly the process of acquisition of biomedical signals, in order to achieve a correct approach when developing diagnostic...

25 March 2019 6,333 4 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out?

Can we patent a process flow diagram developed using a process simulator but no actual cases is carried out? For example consider a process for certain product manufacture where a new process flow...

31 July 2024 781 1 View

Can anyone help me with in providing the procedure for purification of GSTtagged protein by gst affinity chromatography without an automated machine?

Purification of gst tagged protein

25 July 2024 1,720 0 View

Trial exclusion in eye-tracking data?

Is it reasonable to exclude all trials with a blink or saccade in the 150 ms before stimulus onset? As an alternative, would it be better to exclude blinks (after extending them by about 100 ms...

19 July 2024 3,838 0 View

Can anyone suggest me the questionnaire along with scoring key to assess the mental health wellbeing of the college faculty?

I am Ruthra, and I have been working as a psychologist for the past 10 years. Now, I'm interested in conducting research and I would like to conduct a survey on mental health and wellbeing among...

15 July 2024 4,119 4 View

What are the best ways to automate my job and live without working?

I want to know the best methods to automate my job and live without working?

12 July 2024 5,993 2 View

How can get paper ?

I need a copy of the research titled “An Efficient Automated Multi-Modal Cyberbullying Detection Using Decision Fusion Classifier on Social Media Platforms" An efficient automated multi-modal...

08 July 2024 7,381 3 View

What is the current status of augmented learning in robotic surgery?

I would like to perform a literature review at this time on augmented learning and learning augmented algorithms to enhance performance-guided surgery

06 July 2024 246 1 View

How to fill large gaps in GIS?

Dear colleague, I need help with a GIS issue. The polygons have been separated by roads, leaving space between them (as shown in the attached figure). I'm looking for an automated solution in...

04 July 2024 6,752 3 View

Why do you think blockchain is fundamental for digital transformation and role of automation in digital transformation?

02 July 2024 1,735 2 View