I am working on saffron and my project is to in vitro induce flowering in saffron. In literature i didnt find any successful flower induction in this specie instead stigma or stigma like structures are reported. I want to know is there any method that can induce flowering in saffron or let say monocots? (Using different combinations of PGR is the first one but none of them got successful results)
Dear Asif,
Flowering in plants can be induced through in vitro cultures, provided the explant must be mature enough and possess flower induction capabilities, under in vitro cultures, with minimum phytohormones required for promoting shoot growth/somatic cell divisions. In biennials/ perennials, axillary bud explants are ideal, which has to be excised prior to the blooming season of the plant, at least 1 month before. If the plant is a short-duration one (annuals), long-term cultures are required to induce flowering under in vitro conditions. In mulberry, the in vitro flowering achieved the method explained above.
Best Wishes.
Asif Khan,
For in vitro flowering in monocots, we had very good experience in inducing flowering using defined coconut water.
Coconut water contains a good combination of phytohormones and adding them to the right tissue type will induce in vitro flowering consistently.
Defined coconut water refers to our lab approach of collecting around 100 green coconuts, collect the water and then assessing the phytohormone types and concentration. Following which, we add them to the remaining defined medium.
Article The Chemical Composition and Biological Properties of Coconu...
This paper helps you to "define" the phytohormones in coconut water
Article Simultaneous analysis of different classes of phytohormones ...
In Singapore, our National University of Singapore colleagues have developed a highly reproducible system for in vitro flowering of a monocot orchid. If you combined our earlier suggested approach to define the various phytohormone content of the so-called unknown coconut water, you will be able to achieve good reproducibility in your experiments for saffron.
Dear Asif, you can try short-term stress in the presence of sugar. It may help.
Best wishes!
Is there a literature available on techniques for in vitro flowering of bulb or corm flowers at all? If it's possible to induce flowering in a tulip, hyacinth or narcissus surely you could transfer the technology and protocols to a member of the genus Crocus, right?
Jean W H Yong: Thank you so much for yours feedback. I have checked out your papers and found it helpful as earlier i have also found successful flowering using coconut water in other species. I wanted to know if i use another method like transformation by inserting some flower inducing gene from rice or any other specie, Will it give me some results? because it is a monocot and often transformation doesnt succeeded.
Regards
Taras Pasternak: Good idea. that can be checked in parallel
Peter Bernhardt : To my knowledge, there is no literature reported on the in vitro corm flowers though dozens of papers have reported successful induction of floral parts. Regarding bulbs, i have not checked it yet.
@Asif Khan:
could you please tell us about the genes that you have worked with for early flowering in plants?
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