We can screen a rhamnolipid producer by CTAB and haemolysis assays but these assays are not promising if we are screening for hyperproducers. So I need to know methods for selection of general/specific mutants.
Hi,
Heomolytic style assays are best suggested for agar plate assays. Did you try agar plates with lipase enzymes. This paper suggests different method of lipase productivity detection and even quantification. Hope this helps
http://scialert.net/fulltext/?doi=ijbc.2011.116.126&org=10
Some suggestions will be limited without knowing what species, or at least general info about the species if you'd like to leave the name out, is that you are attempting to isolate a mutant strain of. You could move to a broth-based assay and use a property of rhamnolipids as a readout (OD change at a certain wavelength, fluor, stain, ect.). Also, do you have a mutant library to screen? If you don't, can you make one either chemically or Tn-based?
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