Hey All,
Is there any guideline permit or forbid reverse filteration on 0.2 micron liquid filters in sterile manufacturing? Is it the same regarding filter wetting after production or it just for troubleshooting filter integrity testing failing?
Which tools can be used to generate machine codes for additive manufacturing?
02 March 2021 7,220 2 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...
25 February 2021 8,039 1 View
I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....
24 February 2021 4,428 3 View
I am currently trying to stain for PI3KBeta in MDA-MB-231 cells. My current issue is that 1) beta stains all over the cell and 2) there is an increase in signal at the edge of the cell and this...
24 February 2021 8,582 1 View
20 February 2021 8,202 6 View
Most of the references I found were on the economic productivity in agriculture but very limited application in manufacturing sector. I would appreciate if anyone could share the references on...
15 February 2021 8,342 7 View
Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...
14 February 2021 6,247 3 View
Hi guys, I have no experience and little knowledge on immunofluorescence and confocal microscopy. I tried to visualize the expression of FXR in my cells (THP-1 macrophages), yet I did not...
11 February 2021 562 6 View
While running gels for western blotting, I am currently getting, what seems to be, the blue dye from my sample buffer migrating more slowly in sample lanes leaving blue streaks. These transfer to...
08 February 2021 5,317 3 View