I would like to conduct a [1H-13C]- HSQC (optimized for one bond H-C connection) experiment. What would be the best default topspin pulse program and parameters set to use ?
Thank you.
that would really depend on what console and version of topspin you are operating I guess, but I find the basic phase-sensitive version works very well for me with basic acquisition parameters with the added benefit of giving you multiplicity editing (you'll know your CH3/CHs from your CH2s just like in 1D DEPT135) - that one should be available under the name HSQCEDETGP
otherwise, the more classic version: HSQCETGP
these are with echo-antiecho
if not suitable for you, you can also try the HSQCGPPH (normal) and HSQCEDGPPH (phase sensitive) using z-filter
Thank you very much for your answers ! I am using an "Avance Neo 400 MHz" with TopSpin 4.0.7 and a RT-BBFO probehead, if this can help make your answers more specific.
then the pulse sequences I mentioned should all be there - as for optimization of acquisition parameters, it would depend on your sample (molecular weight, concentration, etc), on what you need from the spectrum (more sensitivity or more resolution) and on how much time can you spare for the experiment on the machine)
Commonly, with typical concentrations that give you a good proton spectrum with the routine zg30 in 16 scans, default parameters should be ok (about 15 min).
--> to get better sensitivity if you have a low concentration, increase the NS in the proton dimension (I think that's F2 with usual settings? anyway, the left-most column and the only one with the NS parameter activated)
--> if you need better resolution in the 13C dimension, increase the TD in that column (F1 I think) but if you are unsure of what you are doing beware not to go too far... rule of thumb TD in 13C dimension
Dear Salem,
anything said above is correct - just a minor comment. If your sample has larger line-width (e.g. > 20 Hz) in our hands hsqcetgp (no sensitivity enhancement, no multiplicty edting) sometimes works amazingly well - but I definitely would not recommend it as "THE STANDARD"....
Kind regards
Alfred
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