Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Using Chromotek GFP-Trap Magnetic Agarose beads for Nuclear Protein Purification?

Hi All, I was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins. I am attempting to purify a gfp-tagged protein...

14 January 2020 4,572 2 View

Is the concept of an addictive personality a myth?

I read the relevant wikipedia page and it seems quite intuitive as a construct, but I know that just because something seems to make sense doesn't make it true. Thoughts?

03 August 2019 3,467 4 View

Solutions to selectively break down proteins and leaving polymer chains intact?

In a project, I am required to degrade proteins to a size of 10Kd. This would prevent protein from passing through ultrafiltration and being absent in the resultant. I suggested using SDS,...

27 January 2019 3,300 3 View

Western Blot Signal Looks Like Backround Color?

I am designing an antibody to detect the protein LynA in western blot. Right now I have 8 potential antibodies (SCFVs) that may or may not bind by western blot. I have an apparatus that lets me...

28 November 2018 5,889 1 View

Taqman microRNA Assay Primer dimerisation at exceptionally high levels?

I have been using taqman microRNA assays for 3 years and have recently come across a very serious problem with one particular microRNA. RT-qPCR analysis shows the miRNA to be expressed at higher...

22 November 2018 8,147 3 View

Can I stain intestines with cresyl violet?

Hi everyone, I apologize if this has been asked before. I am running a study with rats and want to collect intestinal samples to look at histologically. Firstly, do the intestines need to be...

18 October 2018 6,288 2 View

Does the populations of argonaute-2 microRNAs and RISC loaded miRNA correlate with total miRNA within cells/tissue?

We've performed Profiling of Ago-2 RIP miRNA in tissue and also followed it with individual RT-qPCRs and showed that the miRNA levels between the 2 were correlated but this is with a handul of...

02 February 2018 1,841 2 View

Can mice reproduce after in utero injections?

I am thinking of doing in utero injections of mice to study the effects of a drug on the embryos. However, we hope to use the female mice again after it gives birth to the injected pups. Is it...

02 January 2017 882 4 View

Why do prices of consumables from the same vendor vary so much between institutions?

Hi, The lab I have been working in recently move from one institution to the other (moved states too!). I noticed that the pricing of consumables varies a whole lot. For instance, in one...

31 December 2015 9,316 0 View

What are some of the research gaps in machine learning and artificial intelligence in africa?

i would to know some of the research gaps in the artificial intelligence field in most african countries.

03 March 2021 6,145 3 View

Is there any Machine Learning/AI algorithm that lets you predict with incomplete features without missing value imputation process?

For instance, the model is trained with A,B,C,D,E features to predict F target. Then, I want to make prediction on a new data, but with only A,C,D,E is known without doing missing value imputation...

28 February 2021 1,556 6 View

What are the important topics in the field of Artificial Intelligence in Business world?

Dear All, I am doing my MS in Electronic Businesses. And I am interested in the AI field and its applications for businesses, its benefits, impacts, etc. What kind of scientific research dominate...

26 February 2021 8,816 6 View

Suggest me some trending topics for phd research in text mining/AI/machine learning?

My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.

24 February 2021 393 14 View

How can we improve AI in detecting fraudulent activities on digital payments?

I am conducting a study on the effectiveness of AI towards detecting and averting digital payment fraud. What are the popular AI techniques being adopted in mitigating digital fraud and how could...

21 February 2021 3,888 4 View

Can anyone suggest a topic for the Impact of AI and ML in the financial institutions?

I am currently doing my Master Thesis in FinTech and I am thinking of doing my project on the impact of AI and ML on the financial industry but as the impact of these technologies on the industry...

21 February 2021 7,128 3 View

Why at farmer level Cow/Buffalo are not done artificial insemination just after 2 month for yearly calving?

Physiologically in cow, oestrous heat again goes on the peak after 2 months but locally this is not observed and AI is after two months but done after at least 6 months.

17 February 2021 685 4 View

What is/are the best ML classifier(s) for Sentinel2 images?

Hi I am trying to segment a sentinel2 image. At this stage, I want to run a binary classifier that assigns each pixel to either farm or non-farm pixel. For this purpose, I have 4 10m bands...

15 February 2021 3,596 9 View

What are the popular techniques for pre-processing measurement data in Smart Grid/Power System?

The data collected from various measurement devices often contains erroneous values - such as noise, missing values, etc. What are the popular techniques for pre-processing measurement data in...

14 February 2021 4,642 2 View

How to convert isotrophic Deriche filter into anistrophic?

12 February 2021 5,889 6 View