01 January 1970 4 983 Report

where eccentricity is constructed as a personality dimension

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Molecular Cloning problems - Weird bands in my ligase sample?

I have been trying to clone a 50bp shRNA gene insert into a plasmid 8.8kb long with compatible sticky ends. For some reason the ligation reaction is not effective and so the transformation is not...

28 August 2022 7,653 3 View

How to conduct a lipase assay using 4-nitrophenyl laurate?

I am currently trying to conduct a lipase assay using 4-nitrophenyl laurate, but I am having problems with the optical density. The concentrations I used are the following: Lipase: 2.0 mg/ml...

13 December 2021 4,114 3 View

Gibson assembly to remove a specific domain, generating two fragments from one template. Is there a better method?

I am designing a cloning experiment to remove a domain of the protein my lab studies using gibson assembly. It seems to me that the overhangs I design into primers targeting the two sides of the...

13 November 2021 5,728 4 View

Can someone help me analyze this IR spectra?

So I was running an IR on a sample that I had thought (or rather hoped) was phenol (hydroxy benzene), but what came out was either trash and noise, or I simply did not have phenol. My professor...

04 October 2021 6,391 3 View

How much of an mRNA UTR to include as an input into mFold?

The mFold input has a limit of 2400nt and while searching for common secondary strucutres around miRNA binding site of mRNA we have come across the issue of mRNAs with extremely long 3' UTRs, up...

27 June 2021 7,986 3 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,804 2 View

Effective methods of estimating ligand binding affinity to a protein active site?

Are there any reasonable ways of estimating the binding affinity in like compounds? For example, could I estimate the binding affinity of both tryptamine and 5-Hydroxytryptamine?

21 March 2020 439 3 View

What are some common challenges and their solutions when designing or implementing a geodatabase?

Recently been asked to describe some challenges when designing or implementing a geodatabase and how the challenges were addressed.

17 March 2020 9,243 3 View

Case Study or Experiment?

Hello. I am attempting to draft a model for producing social and affordable housing units in Ireland based on the model used in Vienna. The methodology I have decided to employ is mixed-use. The...

11 March 2020 6,428 3 View

Using Chromotek GFP-Trap Magnetic Agarose beads for Nuclear Protein Purification?

Hi All, I was wondering if anyone had used the chormotek gfp trap magenetic agarose bead kit for purification of GFP tagged nuclear proteins. I am attempting to purify a gfp-tagged protein...

14 January 2020 4,746 2 View

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Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

What are examples of AI for good projects a teacher can assign to students?

So I am organizing an AI seminar. What are possible AI projects in the AI for good spirit? something the students can do and have an impact?

08 August 2024 9,437 4 View

Self-Organizing Superorganisms—as envisaged by Nenad Sestan (2018)?

The rate of glucose consumption by the neocortex is reduced by over 80% during anesthesia (Sibson et al. 1998), which disables the synapses (Richards 2002) that are inundated by glial tissue (Engl...

08 August 2024 3,118 0 View

How to design human-centered classroom in the age of A.I.?

How to design human-centered classroom in the age of A.I.?

08 August 2024 347 5 View

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

Do experts have journals in the field of artificial intelligence and big data that are not indexed by SCI or EI?

05 August 2024 8,836 2 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

What's the role of IT & AI in Telecommunication Industry?

What's the role of IT & AI in Telecommunication Industry?

05 August 2024 8,264 3 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

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