Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

How can I make a center of mass vs time graph?

I have a trajectory file of about 1000 frames which I have calculated a center of mass for each frame. I would like to use the first frame as a reference and see how much the center of mass...

04 February 2021 5,774 1 View

Can my lysis buffer interfere with the quantification of DNA and RNA using Qubit2.0?

I am doing RNA and DNA extraction using the basic phenol / chloroform / isoamyl method to quantify RNA and DNA in aqueous phase, but first, I do a lysis with buffer (Tris-HCL 1M pH8; EDTA 0.5M pH8...

17 January 2021 1,453 3 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

No title

It was published in the Journal of the American College of Toxicology in 1990, but the author's information does not appear in the journal

29 November 2020 2,554 3 View

Karl Marx vrs Adam Smith, can you see the market illusion associated with each model?

If there are sustainability gaps, then there are market illusions as well as broken circular economic structures. Hence there is a market illusion associated with red socialism/Karl Marx and with...

15 October 2020 1,538 8 View

If the daily (hourly) concentration of PM2.5 is not Gaussian. Is correct apply an arithmetic mean in epidemiological studies for risk assessment?

The distribution of daily PM2.5 concentration (hourly) is bimodal in the city of Coyhaique (South of Chile). Coyhaique is the city more polluted by particulate matter accord the WHO. The major...

11 October 2020 2,006 3 View

What is a green market? What is not a green market?

Despite green market thinking being here formally since 2012 Rio +20 conference finding a definition of a true green market is hard to find even in major publications dealing with green growth and...

11 October 2020 7,951 24 View

Is any of these questions with no answer yet relevant to you?

If any of the questions listed below is interesting to you, please write a comment stating your view or please recommend the question you find interesting to contacts you think may be interested...

09 October 2020 4,482 5 View

Can the green economy and green growth exist without green markets?

In 2012 Ric + 20 we shifted in theory to green markets, green economy and green growth thinking as the three indispensable components of environmentally friendly economic development, yet you do...

07 October 2020 3,424 22 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Tooth extraction and subsequent dental implant placement in Sprague-Dawley rats induce differential changes in anterior digastric myofibre size and ?

01 March 2021 7,254 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View