Alternative method of Permeablization in IL17A assay by flowcytometry?

I am evaluating Th17 cells by staining stimulated cells with IL17A and IFN-Y antibody by flow cytometry. Permeabilization step was done with Cytoperm/cytofix reagents (BD Bisciences) yet and the...

01 December 2020 4,553 1 View

Suggestions how to get Shapefile of India with all 700+ Indian districts?

I need shapefile for all 700+ districts of India. From where I can find the India shapefile with all districts. Also, suggest how to get the latest India shapefile with all state and union...

07 August 2020 3,449 5 View

PSO, ABC or GA, which one to choose while solving Mixed Integer Programming problem?

I am interested to solve a mathematical problem (MILP) using evolutionary algorithms but confuse about which one to choose as a beginner in the programming languages. Suggest an algorithm easy to...

13 July 2020 4,027 51 View

What is the best tool for adding references (in a research paper) within a word file?

What is the best tool for adding references (in a research paper) within a word file

16 June 2020 8,275 9 View

How can I check the viability of RAPD Primers?

Dear Researchers Good day to you I want to ask if we can check our RAPD primers ( both in diluted & lyophilized forms) on agarose gel electrophoresis whether they are degraded or not as our...

24 February 2020 9,172 3 View

How can I develop an ANFIS model for WQI in matlab?

I want to implement the Water quality index in ANFIS. I have used constant output, but not getting the desired output.

06 February 2020 3,394 4 View

Can food supply chains be secured by blockchain diffusion???

Blockchain technology promises to provide complete information security and elimination of intermediary alteration. But can we really full utilize this feature to food supply chain. How can we...

03 May 2019 5,476 2 View

When the unit of analysis is 'supply chain' or 'organization', is it acceptable to take more than one respondent from an organization?

Question related to research methodology

06 March 2019 9,150 7 View

No title

02 May 2018 368 2 View

How to generate UDF in Fluent to measure Droplet concentration at a particular downstream in Turbulent flow?

I don't know how to write UDF. If you know then Please help me..or you can also write the UDE for me and Send me.

22 February 2018 1,883 3 View

What are the harmful effects of these extreme rainfall on the health of newborns? What are the reasons for that?

Extreme rainfall associated with climate change is causing harm to babies in some of the most forgotten places.

01 March 2021 5,647 4 View

Any references about insects or animals that can damage or weathering of rock or fossil material?

Are there any references to insects or animals that can cause damage or weathering to the rock or fossil material? books or paper that would be nice. Thank you very much!

24 February 2021 6,805 3 View

Historical wind data/map?

Hello All, I am looking for the historical wind data/map (mainly the year 2020-2021). I would like to look at the wind value and direction for the specifics locations within the US for the...

18 February 2021 9,543 4 View

Anyone else getting lower than expected values for SSRD from ERA5 Land?

We are using data from ERA5 Land for a preliminary analysis with a GDVM. All other data fields we downloaded are aggreable for the region we are running our tests on, however for Surface Solar...

07 January 2021 8,953 3 View

I need a Wind Power Dataset having wind turbine's output power values and corresponding weather data information: wind speed, pressure, temperature?

The dataset is needed for forecasting wind power output using the weather information. I will be much obliged if anyone can share a relevant dataset or link.

04 January 2021 6,344 2 View

I want to use multilayer feedforward neural network with error backpropagation algorithm. I have just 30 years monthly rainfall data. Is this enough??

I am new to neural networks and want to apply multilayer feedforward neural network with error back propagation algorithm for the rainfall prediction. I have just monthly rainfall data of 30...

28 December 2020 9,755 4 View

How to download future precipitation data for years 2021-2030?

For my future discharge forecast in the calibrated HEC HMS model, i need the precipitation data of the future.How can i get this data in the daily timescale?

21 December 2020 8,928 5 View

How weather based insurance is more effective than yield based?

In Nepal, crop insurance and refunding policy is is area-yield /revenue-based and cost of production based. Farmers are partially following crop insurance in comparison to livestock and other life...

07 December 2020 2,127 1 View

How could you explain that weather is a factor with such contribution in keeping the same level of GHG emissions?

" The IEA report looks at changes in greenhouse gas emissions between 2018 and 2019. It finds that energy-using activities increased in 2019, but the emissions did not increase because of a...

03 December 2020 7,073 3 View

How to determine/select the indices (underlying weather index) of weather-based insurance?

It should not be confused with strike and limit thresholds. In this case, I am emphasizing the basis of the index, such as: -Cumulative, accumulated, deficit and excess rainfall values. -Average,...

29 November 2020 3,084 3 View