How the detection in GC MS increases on dervitization, Explanation required from molecular level? Shall I expect spectra of Derivitized Pharmaceuticals in GC MS same as original compounds? What if the NIST library doesn't confirm the spectra of obtained peak but it contain those major ions of the compound? Can I use major ions out of the obtained spectra for quantification purpose?
If you derivatize a compound for analysis by GC/MSD, you change it's structure so it will not match the library spectra. If you know the chemistry used to derivatize the compound, then you should be able to calculate the new expected mass ion. To quantitate based on this modified compound, use your modified sample(s) to create a new, multi-point calibration curve.
Thanks Bill. Any tool/software to calculate new expected ions of dervitized compound?
Looking for reference mass spectra ? A whole searchable database of mass spectra of pure species of atmospheric interest (molecule and dervivatives) is available for download at the EUROCHAMP datacenter. Check out : https://data.eurochamp.org/data-access/mass-spectra/
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