Hi....I have a thesis in which they estimate Glutathione by using Ellman reagent , in the protocol they used sulfosalicylic acid ....my question is that valid? what is the reason of using this acid? is their a reference for alternatives?
I already used sulfosalicylic acid for glutathione quantification and I did not have the speicific yellow color!!!. So I removed the sulfosalicylic acid then It worked very well.