I want some information about the utility of SPE for PAH's determination in leaf samples. Can I try without them? Has someone has tried it? Any methodological advice is welcome.
Dear Vannini, would you please make it more clear for us, what is your leaf primary extraction method ? Does SPE your direct extraction method? And in which stage you want to use or not to use SPE?
My first step was to extract the samples in acetonitrile and, after centrifugation, run this extract in an HPLC system (UV detector). In this way the determination of the individual PAH's is not very clear. I've never used the SPE but I want to know if they could help me
UV detector is not my first choice to detect PAH from the leaf extract without any cleanup because it will see lots of things that absorb UV in the extract. The better detector is fluorescence detector. To do more cleanup, please use the paper below as a guide line. It does not use the cartridge version of the SPE but the dispersive SPE format (easier to do)
http://www.chem.agilent.com/Library/applications/5990-5452EN.pdf
Tanks! Another question: The use of a fluorescence detector increase also the sensibility?
Because florometer is more selective, it would not see any interference that does not fluorese at the specifica wavelength like PAH, then it will be more sensitive because it has better signal/noise ratio than the non-selective UV detector. Your extract is so dirty already even with sample cleanup. Try not to make your life too complicate.
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