- Sir I isolated Polyhydroxybutyrate(PHB) producing bacteria i.e Bacillus sp. and started extraction of of PHB. Sir I want to do characterizaton of the extracted PHB by using of FTIR,HPLC,GC-MS,LC-MS. So, I want standard for characterization. From Sigma Aldrich which one you will prefer for standard for comparsion with my extacted PHB.
Dear Sir. Concerning your issue about the use of Poly[(R)-3-hydroxybutyric acid] natural origin of Sigma Aldrich as a standard for characterization PHB extracted from Baciilus sp . Of course you can use it as standard. I think the following below link may help you in your analysis:
https://www.sigmaaldrich.com/catalog/product/aldrich/363502?lang=en®ion=SA
Thanks
Dear Shivalkar,
You may try one of the following protocols for PHB determination?
- "Biomass composition and methods for the determination of metabolic reserve polymers in phototrophic sulfur bacteria"
- "Gene regulatory and metabolic adaptation processes of Dinoroseobacter shibaeDFL12T during oxygen depletion"
- "Metabolic network analysis of the marine bacterium Dinoroseobacter shibae" (page 37)
You can obtain PHB from Sigma-Aldrich (http://www.sigmaaldrich.com/catalog/product/aldrich/363502) to generate HPLC standards.Good LuckMichaelhttps://link.springer.com/article/10.1007/BF00877431http://www.jbc.org/content/289/19/13219.full.pdfhttp://scidok.sulb.uni-saarland.de/volltexte/2016/6473/pdf/Dissertation_Annekathrin_Bartsch_Endfassung.pdf
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