Is phytochemical testing i.e. total alkaloid, tannins etc applicable to mushrooms sample?

More Chia-Wei Phan's questions See All

How much 1X TAE buffer should I use in preparing 1.2% agarose gel?

Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...

12 July 2024 2,058 5 View

Why hapolid cells show no growth after yeast tetra dissection?

Greetings to science community, I am operating Kluyveromyces marxianus yeast and want to generate haploid cells from diploid cells. However after 4 day since dissection, I found no growth of...

09 March 2024 8,360 0 View

Two of my papers were on the list of other's profile ?

I found two of my papers were on the list of the profile of Chung-Cheng Huang. I cannot add these two papers to my profile. Identifying a sphenoid sinus fungus ball using a nomogram model(DOI:...

01 May 2023 4,760 0 View

How to create a chart displaying temperature changes over the full simulation in Leonardo ENVI-met?

I am needing to create a chart displaying the temperature change over the full duration of my simulation. I am able to get the chart for a single grid but not for the full grid area.

04 November 2022 1,258 0 View

What is the best approach to analyzing a comparative diagnostic accuracy study with a significant proportion of 'unassessable' results?

The study involves 3 diagnostic methods all performed on the same sample: Test A, Test B, and a reference test. The purpose is to compare Test A to Test B, against the reference. Possible results...

14 March 2022 7,824 4 View

Is there a post hoc test for Generalized Poisson regression with vglm?

Due to Underdispersion of my count data, I am considering a generalized Poisson regression to model the effect of 3 independent variables on the dependent count variable. One of these independent...

02 March 2022 7,896 4 View

Do i need to perform Egger's test for dichotomous outcome?

Hi experts, i am a university student working on my thesis.. I have a dichotomous outcome that i am interested to perform Egger's test to find publication bias but while i was searching online i...

10 February 2022 1,934 5 View

How to specify a Fe-doped (semi-insulating )GaN in Silvaco ATLAS?

I was trying to use SILVACO ATLAS to simulate a GaN HEMT? And there is a semi-insulating GaN layer. Can anyone let me know what I should do with it?? thanks

10 February 2022 8,296 2 View

How do I perform a test for subgroup differences in a pooled meta-analysis of prevalence data?

I am performing a systematic review of prevalence studies. I have pooled prevalence rates using the program MetaXL (double arcsine transformation, random effects model) which gives me a point...

07 February 2022 9,406 7 View

Why the N-polar GaN HEMT is a GaN/AlGaN/GaN structure?

The traditional G-polar consists of AlGaN on top and GaN channel layer at the bottom. However, for the N-polar GaN Hemt, all the discussion or research papers found online show the structure of...

07 January 2022 6,961 0 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

The Bigger You Are, the Harder You Fall (some lessons from Dinosaurs)?

Evolutionary fitness is based on an organism’s ability to adapt rapidly to changing environmental circumstances. Large-bodied mammals have been equipped with large brains (and hence a high...

06 August 2024 4,849 2 View

Are air moisture harvesting technologies effective in combating desertification?

Air moisture harvesting Air water collection devices

06 August 2024 5,473 2 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Does atmosphere affect flow of matter & energy on Earth & flow of energy in biosphere related to the flow of food through a food chain?

How does the atmosphere affect the flow of matter and energy on Earth and flow of energy in the biosphere related to the flow of food through a food chain?

02 August 2024 9,644 0 View

State of art in natural disasters?

Are increasing the costs of disasters in the affected countries.

01 August 2024 1,794 2 View

How can I get my Granzyme B flow cytometry stain to be consistent?

I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...

01 August 2024 7,677 2 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

Posthoc test lettering in JAMOVI?

Does anyone know of a module for the JAMOVI software that is capable of generating mean separations using the classic letters based on post hoc results (e.g., Tukey test)? If, as I believe, such...

31 July 2024 3,333 4 View

What are the roles of innovation in achieving the Sustainable Development Goals (SDG)?

31 July 2024 3,533 2 View

João Paulo Bastos Silva

I guess it is possible. See this paper below:

http://scholarsresearchlibrary.com/JMB-vol2-iss2/JMB-2012-2-2-366-374.pdf

Alok Nahata

Yes it is applicable. You can extract the mushroom sample with different solvents and test the presence of different phytoconstituents.

Regards

Chia-Wei Phan

thank you all :) i will go ahead. one thing about these tests is it uses up up to mgs of extracts? Cause some wild mushrooms are hard to get...nevertheless i guess the weight of the extracts in different test can be scaled down propotionally? regards xx

Mostly u will find polysaccharides and triterpenoids in mushrooms in ample amounts.

thank you for the very helpful suggestions Dr Alok. I appreciate that. Any ideas on alkaloids, flavonoids, anthraquinones, glycosides, tannins, saponins, even steroids? triterpenoids are steroids right?