Hi everyone,
I treated my primary neonatal mouse cardiomyocytes, and observed that under a certain treatment, obvious sarcomeres are seen under microscopy. However, in the control group, such obvious sarcomeres could not be seen. I am wondering whether obvious sarcomere seen under microscopy a sign of cardiomyocyte maturation or any other possible change of cardiomyocytes?
And here attached the figure showing what I observed under microscopy.
Immature cardiomyocytes can show some sarcomere organization. In a mature cardiomyocyte the sarcomere organization is througout the entire cell. Alpha-actinin antibody is used to visualize the extent of Z-disk formation across the cells. It is hard to tell from a phase contrast image alone whether the cells are fully mature.
https://www.nature.com/articles/s41467-021-24049-0
Article Myofibril Assembly in Cultured Mouse Neonatal Cardiomyocytes
https://www.nature.com/articles/s41598-023-31144-3#:~:text=In%20immature%20cardiomyocytes%2C%20sarcomeres%20are,%2DActinin%20(Actn2)%20antibody.
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