Hello, I will start working with HL-1 cells to see if they have my protein of interest, I do not work in cardiac research but it has been difficult for me to find a cell with my protein (already tried 3t3 and 3t3-l1). So I think is not important if the cell contracts or no since I'm not studying hypertrophy. Knowing this I have two questions:
1. Is it essential to supplement the claycomb media with Norephinephrine for cell survival / maintaining the cardiac phenotype ?
2. If yes do you think I could use Norepinephrine bitartrate instead of the (±)-Norepinephrine (+)-bitartrate salt that they speciffically say in the protocol? (is what I have already available in my lab)
Thank you very much for any answers/opinions that you can provide
It's not strictly essential to supplement them with Norephinephrine as they grow and even maintain some level of contraction without it. Although we found that they proliferate faster with the Norephinephrine.
Regarding the second part of the question, I think it wouldn't make a difference.
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