I have a dataset of carbohydrates concentrations measured in Posidonia oceanica (Mediterranean seagrass) leaves with the Dubois methods (ie the phenol sulfuric acid method). Since P. oceanica (just like all marine seagrasses) are terrestrial plant that return to the sea, it is well know that its leaves are full of complex compounds like cellulose which are detected by this method.
Do someone know if it is possible to consider lignin as a carbohydrate, and if it is detected with PSA method ?
Thanks in advance
have a look to the AOAC manual or MERC, there you will find many methods for differenet technology /or manual analysis.
It is not good to consider lignin a carbohydrate as far as it is a polymer of phenols. If it is present in the sample it could affect any measurements done with this method. What one is actually measured is concentration of condensed products from reaction of different furaldehydes with phenol. So when you will have lignin it will react as well and could even lead to precipitation and so the product will escape from analysis or lead to better adsorbens at wave length of measurement.
Lignin is quite different from carbohydrate as it is made up of phenylpropanols. So, better you choose any other mehod for lignin like TAPPI T-222.
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